Journal: Cancer Management and Research
Article Title: DSG2 and c-MYC Interact to Regulate the Expression of ADAM17 and Promote the Development of Cervical Cancer
doi: 10.2147/CMAR.S456548
Figure Lengend Snippet: DSG2 regulates cervical cancer development by interacting with c-MYC. ( A ) HeLa cells were transfected with pcDNA(3.1)-DSG2 overexpression plasmid, and the mRNA level of DSG2 was detected by qPCR. ( B ) HeLa cells were transfected with pcDNA(3.1) overexpression plasmid, and the protein level of DSG2 was detected by WB. ( C and D ) DSG2 overexpressed HeLa cells were treated with a C-MYC inhibitor (10,058-F4, 50 μM), and the proliferative activity and migration ability were detected by CCK-8 ( C ) and clonal formation assay ( D ). ( E ) DSG2 overexpressed HeLa cells were treated with a C-MYC inhibitor, and ADAM17 protein expression was detected by WB.*P < 0.05, **P < 0.01, ***P < 0.001.
Article Snippet: Primary antibodies against DSG2 (21,880-1-AP, 1:1000, Proteintech), ADAM17 (29,948-1-AP, 1:2000, Proteintech) and c-MYC (67,447-1-Ig, 1:5000, Proteintech) were used.
Techniques: Transfection, Over Expression, Plasmid Preparation, Activity Assay, Migration, CCK-8 Assay, Tube Formation Assay, Expressing